Fig. 4: Effect of DUS1L expression on the growth speed of a glioblastoma cell line. | Communications Biology

Fig. 4: Effect of DUS1L expression on the growth speed of a glioblastoma cell line.

From: Human DUS1L catalyzes dihydrouridine modification at tRNA positions 16/17, and DUS1L overexpression perturbs translation

Fig. 4

Growth of DUS1L KO (a) and DUS1L OE (b) cells. The cell lines are the same as those used in Fig. 2. The cells were seeded at the same density at 0 h, and cell density was measured after 24, 48, 72, and 96 h using a Cell Counting Kit 8 and normalized by the cell density at 24 h. Means ± s.e.m. from n = 8 samples at each time point and cell type. ****P < 0.0001, n.s. not significant compared with control cells by a two-way ANOVA followed by a multiple comparison test. c Kaplan–Meier plot of glioma patients with higher or lower levels of tissue DUS1L mRNA expression and the P-value between the two groups (**P = 0.0024) acquired from the Human Protein Atlas database31. The cut-off FPKM value between high and low DUS1L expression was set to 7.47 in order to discern 76 patients with high DUS1L expression and 77 patients with low DUS1L expression.

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