Fig. 5: Effects of DUS1L overexpression on mature tRNA levels and total cellular translation. | Communications Biology

Fig. 5: Effects of DUS1L overexpression on mature tRNA levels and total cellular translation.

From: Human DUS1L catalyzes dihydrouridine modification at tRNA positions 16/17, and DUS1L overexpression perturbs translation

Fig. 5

Mature tRNA levels in DUS1L KO (a) and DUS1L OE (b) cells. Northern blot analysis of tRNATyr(GUA), tRNAPhe(GAA), tRNAAla(AGC), and initiator tRNAMet(CAU) was performed, followed by band quantification and normalization by 5.8S rRNA. The original images are shown in Supplementary Figs. 4 and 5. Means ± s.e.m. from n = 4 samples each. *P < 0.05 by the Mann–Whitney test. c Nascent cellular protein synthesis observed by 35S-methionine pulse labeling. A radiation image of the electrophoresed gels is shown on the left. Coomassie brilliant blue (CBB) staining of the same gel is shown on the right as a loading control. n = 4 samples each. d Quantification of the nascent protein levels in (c). Means ± s.e.m. from n = 4 samples each. *P < 0.05 by the Mann–Whitney test. Exact P-values are included in the Supplementary Data 1.

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