Fig. 7: Effects of DUS1L overexpression on translation of tyrosine codons and translational readthrough of near-cognate stop codons.

a Effects of DUS1L overexpression on translation of tyrosine codons. Cells were transfected with a codon translation reporter plasmid with a 5′ firefly luciferase sequence and a 3′ renilla luciferase sequence connected via five consecutive TAC, TAT, or random codons (control). Plasmid expression was normalized by dividing renilla luciferase luminescence by firefly luciferase luminescence. Possible renilla-to-firefly luciferase translational bias between control and DUS1L OE cells due to codons in luciferase genes was normalized using the random codon linker control. b Effects of DUS1L overexpression on translational readthrough of UAA and UAG stop codons. Cells were transfected with a stop codon readthrough reporter plasmid with a 5′ renilla luciferase sequence and a 3′ firefly luciferase sequence connected via a TAA codon (or its control TTA codon) or TAG codon (or its control TTG codon). Plasmid expression was normalized by dividing firefly luciferase luminescence by renilla luciferase luminescence. Possible firefly-to-renilla luciferase translational bias due to codons in luciferase genes was normalized using the corresponding TTA/TTG linker control. Means ± s.e.m. from n = 6 samples each. **P < 0.01, *P < 0.05 by the Mann–Whitney test. Exact P-values are included in the Supplementary Data 1.