Fig. 3: Colocalization between replication and transcription in U937-PR9 from confocal images. | Communications Biology

Fig. 3: Colocalization between replication and transcription in U937-PR9 from confocal images.

From: Super-resolved analysis of colocalization between replication and transcription along the cell cycle in a model of oncogene activation

Fig. 3

a Representative 3-color confocal image of U937-PR9 cells labeled with EdU (green), Pol2 (red), and DAPI (blue). Scale bar 10 µm. b Representative images of replication (EdU) and transcription (Pol2) foci in U937-PR9 cells sorted into diffferent S subphases (Early, Middle, Late). Scale bar 5 µm. c Colocalization fraction between replication and transcription extracted by ICCS (Early, f1 = 0.63 ± 0.07, mean ± s.e.m., n = 13 cells; Middle, f1 = 0.08 ± 0.01, mean ± s.e.m., n = 207 cells; Late, f1 = 0.03 ± 0.22, mean ± s.e.m., n = 2 cells). Mann–Whitney test was performed for statistical significance. d Schematic representation of the meaning of the ICCS colocalization fraction extracted from confocal images, in relation to the distribution of the replication and transcription foci.

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