Fig. 4: VapBC13 and VapBC26 TA systems are dispensable for the in vitro growth of M. tuberculosis. | Communications Biology

Fig. 4: VapBC13 and VapBC26 TA systems are dispensable for the in vitro growth of M. tuberculosis.

From: Deciphering the role of VapBC13 and VapBC26 toxin antitoxin systems in the pathophysiology of Mycobacterium tuberculosis

Fig. 4

Schematic representation of vapC13 (A), vapBC13 (B), vapC26 (C) and vapBC26 (D) TA loci in wild type and respective mutant strains. The construction of ΔvapC13 (E), ΔvapBC13 (F), ΔvapC26 (G) and ΔvapBC26 (H) was confirmed by Southern blot. I–L The growth pattern of parental and various mutant strains was compared by measuring OD600nm at regular intervals. The overexpression of VapC13 (M) or VapC26 (N) using an anhydrotetracycline inducible vector inhibits the growth of ΔvapBC13 and ΔvapBC26 strain, respectively. The data shown in panels I–N is representative of two independent experiments. Quantitative real-time PCR was performed to determine the expression levels of a subset of DEGs obtained upon VapC13 (O) or VapC26 (P) overexpression in the ΔvapBC13 or ΔvapBC26 mutant background, respectively. The data obtained was normalized to sigA levels and is shown as mean ± SD of log2 of fold change obtained from three independent experiments. Source data is provided in Supplementary Data Set 4.

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