Fig. 3: USP24 increased the level of autophagy. | Communications Biology

Fig. 3: USP24 increased the level of autophagy.

From: USP24 promotes autophagy-dependent ferroptosis in hepatocellular carcinoma by reducing the K48-linked ubiquitination of Beclin1

Fig. 3

A The relationship of USP24 expression with autophagy markers was investigated via Gene Expression Profiling Interactive Analysis (GEPIA). B qRT-PCR was performed to measure the mRNA expression of ULK1, Atg5 and Beclin1 after silencing USP24 (n = 3). C qRT-PCR was performed to measure the mRNA expression of ULK1, Atg5 and Beclin1 after overexpressing USP24 (n = 3). D The expression of ULK1, Atg5, Beclin1, LC3B-II and p62 proteins in USP24 knockdown HCC cells were measured by western blot. E The expression of autophagy related proteins after overexpressing USP24 was detected by western blot. F Transmission electron microscopy (TEM) was used to detect the formation of autophagic structures. Red arrows denote representative autolysosome. Scale bar, 5 μm (left), 500 nm (right). G The number of autophagic vesicles was counted and presented in the image (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.

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