Fig. 2: DUTs are newly defined cryptic transcripts located mainly within genes.
From: Spt5 orchestrates cryptic transcript suppression and transcriptional directionality
a Metagene profiles show the median intensity obtained under Spt5p KD for the PRO-seq signal in the 3’ end of up-regulated (n = 1164) and down-regulated (n = 476) DUTs. b Pie graphs illustrate the number of up- and down-regulated DUTs belonging to each genomic region. c Bar graphs illustrate the number of transcripts overlapping with DUTs for the following cryptic transcript types; CUT (Cryptic Unstable Transcript), SUT (Stable Unannotated Transcript), SRAT (Set2-Repressed Antisense Transcript), SRT (Ssu72-Restricted Transcript), ncRNA (non-coding RNA), and XUT (Xrn1-sensitive Unstable Transcript). d Boxplots depict the log2FC of the PRO-seq obtained under Spt5p depletion for each cryptic transcript. The number at the top of the boxplot presents the median value of log2FC. e Median plots depict the intensity of the PRO-cap signal from the CSS (Cryptic TSS) in the New Cap (n = 1982), Up Cap (n = 3682), and Down Cap (n = 22) groups. The sequence logos of the CSS in each group are illustrated. The logos for cryptic initiation sites that overlapped with filtered genes are depicted next to the metagene plot.
