Fig. 2: Screening protocol for polydactyly candidate genes based on half-sib family.
From: A dominant missense variant within LMBR1 related to equine polydactyly
a Three offspring were obtained by crossing an eight-digit stallion on the leftmost side of the figure with three mares in the center of the figure. Two colts were mutants. b The variant sites obtained from resequencing were screened in one round according to the inheritance model, and the variants obtained from the screening were then intersected with genes related to polydactyly with high homology in other animals to finally obtain equine polydactyly candidate genes. Diagram of gene screening, with gray circles representing the number of genes that conformed to the inheritance modes, and the lake blue color indicates the number of variants finally screened.
