Fig. 3: MVA pathway inhibition by ZOL affects the proliferation and metastasis of BLCA cells in vivo and in vitro. | Communications Biology

Fig. 3: MVA pathway inhibition by ZOL affects the proliferation and metastasis of BLCA cells in vivo and in vitro.

From: Mevalonate pathway inhibition reduces bladder cancer metastasis by modulating RhoB protein stability and integrin β1 localization

Fig. 3

a Schematic representation of MVA pathway inhibition by ZOL. b An MTT assay was performed to detect changes in the proliferation of BLCA cells (UM-UC-3, T24 and 5637) after treatment with different concentrations (0, 1, 2, 5, 10, 20, 40, 60 and 80 μM) of ZOL for 48 h (n = 6). Representative images (c) and statistical analysis (d and e) of colony formation assays from the indicated groups after treatment with ZOL at different concentrations (0, 5 and 10 μM) in UM-UC-3 cells (d) and T24 cells (e) for 48 h (n = 3). The scale bar is 1 cm. Representative images (f) and statistical graph (g and h) of transwell assays from the indicated groups after treatment with ZOL at different concentrations (0, 10 and 20 μM) in UM-UC-3 cells (g) and T24 cells (h) for 48 h (n = 3). The scale bar is 200 μm. i Western blot analyses of EMT-related proteins in ZOL-treated UM-UC-3, T24 and 5637 cells. GAPDH was used as the loading control. j Schematic representation of the effects of ZOL-mediated inhibition of the MVA pathway on BLCA proliferation and metastasis in a mouse xenograft model and pulmonary metastasis model. k Gross view of a subcutaneous tumor; the upper side represents the control group (n = 3), while the lower side represents the ZOL treatment group (n = 3). l Tumor volume was calculated during the experiment. Images of lung fluorescence in the control group and ZOL treatment group (m) and quantification of the fluorescence intensity of lung metastases (n = 3) (n). Images of dissected whole lungs (o) and representative images of H&E-stained mouse lung tissue sections (n = 3) (p); the scale bars are 4 mm and 100 μm. Statistical analysis of the number of metastatic nodules in H&E-stained mouse lung tissue sections (q). The n number represents n biologically independent experiments in each group. Statistical significance was ascertained by two-tailed unpaired Student’s t-tests (l, n and q) and one-way ANOVA with Dunnett’s multiple comparisons test (d, e, g, and h). The data are shown as the mean ± SD.

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