Fig. 5: Geranylgeranylated RhoB protein is more susceptible to degradation through ubiquitination pathway. | Communications Biology

Fig. 5: Geranylgeranylated RhoB protein is more susceptible to degradation through ubiquitination pathway.

From: Mevalonate pathway inhibition reduces bladder cancer metastasis by modulating RhoB protein stability and integrin β1 localization

Fig. 5

Western blot analysis of the effect of ZOL on RhoB degradation in BLCA cells (T24 and 5637) incubated with CHX (50 μg/mL) for the indicated durations (a) and statistical analysis (n = 3) (b). c BLCA cells (T24 and 5637) with or without ZOL were treated with CQ (50 μM) or MG132 (10 μM) for 8 h, after which RhoB protein expression was detected via western blotting. T24 (d) and 5637 (e) cells with or without ZOL were treated with CQ (50 μM) or MG132 (10 μM) for 8 h, and then ubiquitination experiments were performed to analyze the polyubiquitination of RhoB. T24 (f) and 5637 (g) cells with or without ZOL (20 μM) were treated with GGPP (5 μM) for 24 h, after which ubiquitination experiments were performed to analyze the polyubiquitination of RhoB. h 293 T cells were transfected with the described plasmids for 48 h and then treated with MG132 (10 μM) for 8 h, after which ubiquitination experiments were performed.

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