Fig. 7: RhoB is involved in the translocation of integrin β1 from the cytoplasm to the membrane in BLCA cells. | Communications Biology

Fig. 7: RhoB is involved in the translocation of integrin β1 from the cytoplasm to the membrane in BLCA cells.

From: Mevalonate pathway inhibition reduces bladder cancer metastasis by modulating RhoB protein stability and integrin β1 localization

Fig. 7

Pathway enrichment analysis of DEGs between FDPS knockdown T24 cells and siNC T24 cells (a) and differentially expressed proteins between simvastatin-treated T24 cells and control T24 cells (b). c The integrin β1 and β3 proteins in the cytoplasm and on the membrane of BLCA cells transfected with vector or RhoB plasmid were detected via western blotting. GAPDH and ATP1A1 were used as a loading control for the cytoplasmic and membrane proteins, respectively. d The integrin β1 and β3 proteins in the cytoplasm and on the membrane of BLCA cells treated with different concentrations of these agents (0, 10 and 20 μM) were detected via western blotting. GAPDH and ATP1A1 were used as a loading control for the cytoplasmic and membrane proteins, respectively. e The expression and localization of integrin β1 (red) or RhoB (green) in UM-UC-3 and T24 cells transfected with vector or RhoB plasmid were detected by immunofluorescence staining. Nuclei were stained with DAPI (blue). The scale bar is 25 μm. f The expression and localization of integrin β1 (red) and RhoB (green) in T24 cells treated with ZOL (20 μM) or simvastatin (5 μM) were detected via immunofluorescence staining. Nuclei were stained with DAPI (blue). The scale bar is 25 μm.

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