Table 4 SPR data comparison of single/multiple RBD mutations’ impact onto RBD binding to hACE2 (for mutations in non-RBM regions)

From: Interfacial subregions of SARS-CoV-2 spike RBD to hACE2 affect intermolecular affinity by their distinct roles played in association and dissociation kinetics

Residue Mutated

Ka(*105 M−1s−1) ± SD

Kd(10−3 s−1) ± SD

KD (nM) ±SD

Affinity change

KD(Wild type/Omicron)/KD(RBD mutant)

G339D

2.08 ± 0.04

10.64 ± 0.17

51.02 ± 0.48

↓

0.86

S371F

1.93 ± 0.02

10.36 ± 0.3

53.68 ± 1.23

↓

0.82

S371L

2.2 ± 0.39

11.35 ± 1.92

51.56 ± 0.42

↓

0.85

S373P

2.09 ± 0.38

11.75 ± 1.91

56.5 ± 1.65

↓

0.78

S375F

1.51 ± 0.3

13.07 ± 2.31

86.6 ± 2.42

↓

0.51

T376A

2.07 ± 0.03

10.26 ± 0.07

49.64 ± 0.5

↓

0.89

D405N

2.21 ± 0.03

22.74 ± 0.16

102.74 ± 0.97

↓

0.43

R408S

1.08 ± 0.01

13.21 ± 0.29

121.93 ± 3.16

↓

0.36

N440K

3.89 ± 0.08

9.29 ± 0.04

23.9 ± 0.36

↑

1.84

S371L + S373P + S375F

1.57 ± 0.43

13 ± 3.12

83.27 ± 2.66

↓

0.53

SARS2-RBD-SARS-RBM

3.26 ± 0.38

28.9 ± 3.9

88.63 ± 1.82

↓

0.50

SARS2.Omicron-RBD-F375*

5.59 ± 0.57

3.31 ± 0.31

5.95 ± 0.41

↑

2.00

  1. Selected SARS-CoV-2 RBD single mutants or mutant combinations are located in non-RBM region of RBDs from Omicron B.1.1.529 or BA.5.2. The mutations’ impact onto binding affinity of SARS-CoV-2 RBD/hACE2 interaction were measured by binding affinity change relative to WT SARS-CoV-2 RBD, with enhancement degree quantitatively labelled by ↑, ↑↑, ↑↑↑, ↑↑↑↑ representing 1∼5, 5∼10, 20∼100, >100 fold change respectively, or attenuation degree indicated by ↓, ↓↓, ↓↓↓, ↓↓↓↓ representing 1∼5, 5∼10, 20∼100, >100 fold change respectively. N/A, data not available. For SARS2.Omicron-RBD-375S mutant (B.1.1.529), the binding affinity change was relative to SARS-CoV-2 Omicron-RBD (B.1.1.529) and marked with star symbol.
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