Fig. 4: Measurement of key enzyme activities and PDH bypass gene expression after 72 h of fermentation in ethanol medium. | Communications Biology

Fig. 4: Measurement of key enzyme activities and PDH bypass gene expression after 72 h of fermentation in ethanol medium.

From: Regulating the metabolic flux of pyruvate dehydrogenase bypass to enhance lipid production in Saccharomyces cerevisiae

Fig. 4

A Mitochondrial ALDH enzyme activity (n = 3 biological replicates, ***p < 0.001, **p = 0.002), ALDH acetaldehyde dehydrogenase. B Cytoplasmic ALDH enzyme activity (n = 3 biological replicates, n.sp = 0.180, n.sp = 0.917, n.sp = 0.209). C ADH enzyme activity (n = 3 biological replicates, ***p < 0.001, **p = 0.002), ADH alcohol dehydrogenase. D PDC enzyme activity (n = 3 biological replicates, **p = 0.002), PDC pyruvate decarboxylase. E Acetyl-CoA content (n = 3 biological replicates, ***p < 0.001, **p = 0.009). F qPCR of ALDH-encoding genes. G qPCR of ADH-encoding genes. H qPCR of PDC-encoding genes. I qPCR of ACS-encoding genes; ACS acetyl-CoA synthetase. J qPCR of other genes. Error bars represent standard error (SE).

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