Fig. 2: Functional validation of FeSig genes. | Communications Biology

Fig. 2: Functional validation of FeSig genes.

From: Ferroptosis-related gene signature for predicting prognosis and identifying potential therapeutic drug in EGFR wild-type lung adenocarcinoma

Fig. 2

a Western blot analysis of knocking down (KD) of DDIT4, NOX4 and GCLC in A549 and H441, respectively. b Cell viability analysis (Student’s t test, two-tailed). c Clone formation assay of control A549 and A549 with DDIT4, NOX4 and GCLC knocking down, respectively. d Quantitative analysis of clone formation assay of A549 (Student’s t test, two tailed). e Scratch assay of control A549 and A549 with DDIT4, NOX4 and GCLC knocking down, respectively. f Quantitative analysis of migration rate of A549 and corresponding gene silenced group (Student’s t test, two-tailed). g Flowcytometry assay of ferroptosis detection after RSL3 treatment (Student’s t test, two-tailed). Error bars depict as mean ± SD.

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