Fig. 1: CHIP was downregulated in cisplatin-induced AKI. | Communications Biology

Fig. 1: CHIP was downregulated in cisplatin-induced AKI.

From: CHIP drives proteasomal degradation of NUR77 to alleviate oxidative stress and intrinsic apoptosis in cisplatin-induced nephropathy

Fig. 1

A RPKM value analysis showed that CHIP had the highest value than other U-box E3 ligases in human or mouse kidneys. B, C qRT-PCR data confirmed that CHIP had the highest mRNA levels in BUMPT cells and mouse kidneys, as compared with other U-box E3 ligases. D Immunofluorescence staining showed that CHIP (green) co-stained with MEGALIN (red, a marker of proximal tubules), suggesting that CHIP is highly expressed in proximal renal tubular cells of mouse kidneys. E Immunofluorescence staining showed that the expression of CHIP was decreased in cisplatin-treated BUMPT cells. F Immunohistochemistry staining indicated that CHIP was downregulated in cisplatin-treated mouse kidneys. G, H Western blot studies revealed that CHIP was downregulated in cisplatin-treated BUMPT cells and mouse kidneys. (n = 3 *P = 0.0023 vs. the WT BUMPT cells; n = 3 *P = 0.0269 vs. the WT mouse kidneys) Scale bars: 50 μm, Error bars show SD, WT: wide type, CP: cisplatin.

Back to article page