Fig. 2: CHIP overexpression alleviated cisplatin-induced BUMPT cell injury. | Communications Biology

Fig. 2: CHIP overexpression alleviated cisplatin-induced BUMPT cell injury.

From: CHIP drives proteasomal degradation of NUR77 to alleviate oxidative stress and intrinsic apoptosis in cisplatin-induced nephropathy

Fig. 2

A Light microscopy showed that cisplatin-induced cellular shrinkage, which was ameliorated by the CHIP overexpression. TUNEL staining revealed that CHIP overexpression attenuated cisplatin-induced DNA damage in BUMPT cells. DHE and DCF staining demonstrated that cisplatin-induced increase in ROS generation was mitigated by CHIP overexpression. B Western blot studies related to CHIP and FLAG confirmed lentivirus-mediated overexpression of CHIP in BUMPT cells. C Western blotting studies showed that CHIP overexpression attenuated cisplatin-induced upregulation of cleaved-caspase 3 and BAX and downregulation of BCL2 in BUMPT cells. D Quantification of TUNEL staining. (n = 3 *P = 0.0002 vs. the Lv-NC group.) E Quantification of DCF staining by flow cytometry. (n = 3 *P = 0.0117 vs. the Lv-NC group.) F MTT assay revealed that CHIP overexpression increased the cell survival in cisplatin-treated BUMPT cells. (n = 5 *P < 0.0001 vs. the Lv-NC group.) WT: wide type, CP: cisplatin, Lv-NC: empty vector lentivirus for CHIP overexpression, Lv-CHIP: overexpression of CHIP by lentivirus. Scale bars: 100 μm, Error bars show SD.

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