Fig. 3: CHIP knockdown aggravated cisplatin-induced BUMPT cell injuries. | Communications Biology

Fig. 3: CHIP knockdown aggravated cisplatin-induced BUMPT cell injuries.

From: CHIP drives proteasomal degradation of NUR77 to alleviate oxidative stress and intrinsic apoptosis in cisplatin-induced nephropathy

Fig. 3

A Light microscopy showed that CHIP knockdown accentuated cisplatin-induced cellular morphological shrinkage. TUNEL staining indicated that CHIP knockdown enhanced cisplatin-induced DNA damage in BUMPT cells. DCF and DHE staining demonstrated that CHIP knockdown accentuated cisplatin-induced increase in ROS generation. B Western blot studies demonstrated lentivirus-mediated downregulation of CHIP in BUMPT cells. C Western blot studies showed that CHIP knockdown accentuated cisplatin-induced upregulation of cleaved caspase 3 and BAX and downregulation of BCL2 in BUMPT cells. D Quantification of TUNEL staining. (n = 3 *P = 0.0002 vs. the shNC group.) E Quantification of DCF staining by flow cytometry. (n = 3 *P = 0.0069 vs. the shNC group.) F MTT assay revealed that CHIP knockdown exacerbated cisplatin-induced BUMPT cell death. (n = 5 *P < 0.0001 vs. the shNC group.) WT: wide type, CP: cisplatin, shNC: empty vector lentivirus for CHIP knockdown, shCHIP: knockdown of CHIP by lentivirus. Scale bars: 100 μm, Error bars show SD.

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