Fig. 4: CHIP overexpression mitigated cisplatin-induced AKI. | Communications Biology

Fig. 4: CHIP overexpression mitigated cisplatin-induced AKI.

From: CHIP drives proteasomal degradation of NUR77 to alleviate oxidative stress and intrinsic apoptosis in cisplatin-induced nephropathy

Fig. 4

A H & E staining revealed that CHIP overexpression mitigated cisplatin-induced morphological changes (including tubular dilatation, cast formation, and interstitial edema) in mouse kidneys. DHE staining showed that CHIP overexpression alleviated cisplatin-induced increased ROS generation in renal tubules. B CHIP overexpression alleviated cisplatin-induced increase in serum creatinine levels. (n = 5 *P < 0.0001 vs. the Ad-NC group; *P = 0.043 vs. the Ad-CHIP group; #P < 0.0001 vs. the Ad-NC + CP group.) C CHIP overexpression alleviated cisplatin-induced increase in serum BUN levels. (n = 5 *P = 0.028 vs. the Ad-NC group; *P < 0.0001 vs. the Ad-CHIP group; #P = 0.0031 vs. the Ad-NC + CP group.) D qRT-PCR data showed that cisplatin-induced increase in the mRNA levels of KIM1 was mitigated by CHIP overexpression. (n = 4 *P < 0.0001 vs. the Ad-NC group; *P = 0.0009 vs. the Ad-CHIP group; #P < 0.0001 vs. the Ad-NC + CP group.) E qRT-PCR data showed that cisplatin-induced increase in the mRNA levels of NGAL was mitigated by CHIP overexpression. (n = 4 *P < 0.0001 vs. the Ad-NC group; *P < 0.0001 vs. the Ad-CHIP group; #P < 0.0001 vs. the Ad-NC + CP group.) F Western blot studies confirmed that CHIP was overexpressed in Ad-CHIP adenovirus-treated kidneys. G Western blot studies revealed that CHIP overexpression attenuated cisplatin-induced upregulation of cleaved caspase 3 and BAX, and downregulation of BCL2 in kidneys. CP: cisplatin, Ad-NC: empty vector for CHIP overexpression, Ad-CHIP: overexpression of CHIP by adenovirus. Scale bars: 100 μm, Error bars show SD.

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