Fig. 2: scRNAseq analysis of WK2 and WK4 SEAMs. | Communications Biology

Fig. 2: scRNAseq analysis of WK2 and WK4 SEAMs.

From: Single-cell transcriptomics reveals the molecular basis of human iPS cell differentiation into ectodermal ocular lineages

Fig. 2

a Experimental timeline and representative phase-contrast image showing WK2 SEAMs in culture. Scale bar: 5000 μm; inset, 500 μm. b UMAP plot of WK2 SEAMs. c Dot plots showing expression of lens progenitor cell markers FOXE3, BHMT, PITX3, PDGFRA and CRYAB. d Dot plots illustrating overlapping expression domains of NR markers and RPE marker MITF. e Dot plots showing expression of glial and neural markers. f Feature plots showing populations of stem (POU5F1, ABCG2) and surface ectoderm-derived epithelial cells. Elevated PAX6 expression indicates ocular lineages. g Experimental timeline and representative phase-contrast image showing WK4 SEAMs in culture. Scale bar: 5000 μm; inset, 500 μm. h UMAP plots of WK4 SEAMs. i Feature plots showing widespread expression of PAX6, SIX6 and SFRP2 and reciprocal expression of VSX2, SOX2 and RAX in developing NR compared with MITF and PMEL in RPE. j Lens marker expression in Seurat cluster 12. k Violin plots showing contribution of neuronal cell subtypes to clusters 4 and 5. Z1 Zone 1, Z2 Zone 2, Z3 Zone 3, Z4 Zone 4, SF StemFit medium, DM differentiation medium, CDM corneal differentiation medium, CEM corneal epithelium maintenance medium, PSC pluripotent stem cell, PC progenitor cell, RPC retinal progenitor cell, NR neural retina, RPE retinal pigment epithelium. ‘i‘ denotes immature cells.

Back to article page