Fig. 1: Correlation based Single Molecule Localization Microscopy (corrSMLM). | Communications Biology

Fig. 1: Correlation based Single Molecule Localization Microscopy (corrSMLM).

From: Fortunate molecules boost signal to background ratio and localization precision in correlation based single molecule localization microscopy

Fig. 1

A graphical representation of corrSMLM and related key steps (optical and computational). The steps involve, spot detection, pair identification, correlation, Gaussian fitting and parameters estimation (localization precision, position and number of emitted photons), and finally, reconstruction of corrSMLM image. Detailed description of the reconstruction can be found in Supplementary Note 1.

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