Table 1 Estimation of critical biophysical parameters (mean localization precision, signal-to-background ratio, number of emitted photons per molecule and total number of molecules) for SMLM and corrSMLM
Technique | organelle | SMLM | corrSMLM | ||
|---|---|---|---|---|---|
0.7 | 0.8 | 0.9 | |||
Mean Loc. Prec. | Actin | 33 | 15.3 | 15.3 | 15.2 |
(nm) | Tubulin | 31 | 17.8 | 17.6 | 17.4 |
Mitochondria | 33 | 20.9 | 20.3 | 19.4 | |
SBR | Actin | 2.44 | 3.14 | 4.03 | 3.83 |
Tubulin | 3.4 | 3.4 | 4.2 | 4.5 | |
Mitochondria | 2.46 | 4.57 | 4.8 | 4.75 | |
#N/Mol. (mean) | Actin | 198 | 534 | 542 | 576 |
Tubulin | 290 | 790 | 802 | 830 | |
Mitochondria | 236 | 784 | 815 | 891 | |
#Molecules | Actin | 93076 | 10261 | 9759 | 8177 |
Tubulin | 25130 | 3379 | 3193 | 2718 | |
Mitochondria | 112879 | 7552 | 6677 | 4925 |