Fig. 5: Rotational movement of tracked loci.

a MSD as in Fig. 4a, b but along the cell’s angular axis. Trajectory angles (in radians) were multiplied with their corresponding radii to calculate the arc length before the MSD estimation. b Selected examples of YZ trajectories and long axis trajectories from time-lapse experiments on the minute time-scale that show rotational or confined diffusion, overlaid on yz-plane location distributions. c As in Fig. 5b, but with trajectories in blue and black corresponding to locus copies from the same cell. Trajectories in blue were in the cell half closest to the new pole and trajectories in black were in the cell half closest to the old pole. The yz-plane location distributions are the same as in Fig. 3b. d Histogram of Pearson correlation coefficients estimated between yz-plane trajectory angles (in radians) for locus copies from the same cell. The histogram was based on tracking 51 locus pairs.