Fig. 3: The social stress induces interconnections between the S1-Tr cortex and the auditory cortex. | Communications Biology

Fig. 3: The social stress induces interconnections between the S1-Tr cortex and the auditory cortex.

From: Associative memory cells of encoding fear signals and anxiety are recruited by neuroligin-3-mediated synapse formation

Fig. 3

A Shows a low magnification image in a coronal section of the brain from C57 mice with the microinjection of AAV2/8-CMV-GFP as an anterograde tracer in the S1-Tr cortex under a confocal microscopy. B Shows sample images about the anterograde projection of GFP-labeled axonal boutons in the auditory cortex from a control mouse and an intruder mouse. Yellow, YFP from Glutamatergic cells; green, AAV2/8-CMV-GFP. C Shows the statistical analyzes about the densities of GFP-labeled axon boutons in the auditory cortex from the mice in control group (blue bar) and intruder group (red bar; mean ± SEM, students’ t test, ****p < 0.0001). D Shows a low magnification image in a coronal section of the brain from C57 mice with the microinjection of AAV2/8-CMV-tdTomato as an anterograde tracer in the auditory cortex under a confocal microscopy. E Shows sample images about the anterograde projection of tdTomato-labeled axonal boutons in the S1-Tr cortex from a control mouse and an intruder mouse. Yellow, YFP from Glutamatergic cells; red, AAV2/8-CMV-tdTomato. F Shows statistical analyzes about the densities of tdTomato-labeled axon boutons in the S1-Tr cortex from the mice in control group (blue bar) and intruder group (red bar; mean ± SEM, students’ t test, ***p < 0.001).

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