Fig. 8: Neuroligin-3 knockdown in the S1-Tr cortex prevents for the formation of new synapses and the recruitment of associative memory neurons. | Communications Biology

Fig. 8: Neuroligin-3 knockdown in the S1-Tr cortex prevents for the formation of new synapses and the recruitment of associative memory neurons.

From: Associative memory cells of encoding fear signals and anxiety are recruited by neuroligin-3-mediated synapse formation

Fig. 8

A Illustrates the confocal images of the coronal section of the brain from C57 mice with the microinjections of AAV2/8-CMV- tdTomato into the auditory cortex (left panel), AAV2/8-CMV-BFP into the thalamus (middle panel) and AAV2/8-CMV-GFP-scramble or AAV2/8-CMV-GFP-shNLGN3 into the S1-Tr cortex (right panel). B Shows sample images of S1-Tr cortical neuron dendrites in each group. A neuronal dendrite in the intruder + scramble group (top panel) receives convergent synapse innervations from the auditory cortex (red boutons) and the thalamus (blue boutons), whereas a dendrite in intruder + shNLGN3 group (bottom panel) receives no synapse contact. C Shows the statistical comparisons of spine numbers of S1-Tr cortical neurons from the mice in the groups of intruder + scramble (purple bar) and intruder +shNLGN3 group (green bar; mean ± SEM, students’ t test, ****p < 0.0001). D Shows the statistical analyzes about synapse contacts from the auditory cortex per 100 μm dendrite in the S1-Tr cortices from the mice of intruder + scramble group (purple bar) and intruder +shNLGN3 group (green bar; mean ± SEM, students’ t test, *p < 0.05). E Shows the statistical analyzes about synapses contacts from thalamus per 100 µm dendrite in the S1-Tr cortices from the mice of intruder + scramble group (purple bar) and intruder +shNLGN3 group (green bar; mean ± SEM, students’ t test, **p < 0.01).

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