Fig. 6: Small hydrophobic substitutions of Orai1 residues C143 and F253 located in the same membrane plane as V181K significantly reduce Orai1-V181K currents.
From: Water in peripheral TM-interfaces of Orai1-channels triggers pore opening
A Schematics showing side and top views of the TM3/TM4-interface along with TM2′ of the next subunit with the tested residues K181, C143 and F253. Time courses of Ca2+ current densities after whole-cell break-in of Orai1-V181K-F253A/W (B) and Orai1-C143A/F/L/W-V181K (D) compared to Orai1-V181K (indicated by /) in the absence of STIM1. C, E Block diagrams of maximal whole-cell current densities of mutants recorded in (B, D). For the presented bar diagrams, ANOVA (one-way) or Welch-ANOVA was employed for statistical analyses with differences considered statistically significant at p < 0.05 indicated with asterisks (see Supplementary Data 1).
