Fig. 2: Histone H2a fragments are KDM5 and KDM4 substrates. | Communications Biology

Fig. 2: Histone H2a fragments are KDM5 and KDM4 substrates.

From: JmjC catalysed histone H2a N-methyl arginine demethylation and C4-arginine hydroxylation reveals importance of sequence-reactivity relationships

Fig. 2

MS analyses [MH]+ reveal −14 Da mass shifts relative to H2a(1–20)R3me2a indicating demethylation on incubation with (a) KDM5A-D (90 min, red), (b) KDM4A–C/KDM4E (60 min, red). b, c A +16 Da mass shift occurs with H2a(1–20)R3me2a, H2a(1–20)R3me1 and H2a(1–20) on KDM4E incubation. *Low level demethylation (<10%). See Supplementary Table 6 and Supplementary Fig. 2 for assay conditions and quantification, respectively. Note in the case of KDM4D, low levels of RDM / hydroxylation activity were observed with different assays (Supplementary Figs. 1 and 6). 0-minute MS spectrum in black, 60/90-minute spectrum in red.

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