Fig. 4: Arginine hydroxylation by KDM4E occurs at C-4 of H2a Arg-20.

NMR analyses of unmodified and KDM4E-treated H2a(1–20). (a) ¹H NMR spectra of unmodified (black) and hydroxylated H2a (red). Note the new multiplet at ~3.2 ppm in the latter (blue), assigned as one of the 5-Hs of C-4 hydroxylated R20. (b) Overlay of HSQC spectra of unmodified (black) and hydroxylated H2a (red) and 1D TOCSY spectrum of the hydroxylated H2a (irradiation at H5); the peak assigned as hydroxylated C-4 is at ¹H 3.8 ppm, ¹³C: 67 ppm. *Residual buffer and glycerol. Supplementary Fig. 11 is a ¹H-¹H COSY spectrum of hydroxylated H2a(1–20).