Fig. 1: DNRX in muscle and neurons is required for Drosophila locomotion.
a Examples of the crawling trajectories of Drosophila larvae of the indicated genotypes over 3 min. Scale bar, 10 mm. b The distance traveled by larvae of the indicated genotypes in 3 min. n = 20, 21, 17, 20, 17, 20, 17 for the different genotypes from w1118 to UAS-dnrx,dnrx273. The data are presented as the means ± SEMs. ****p < 0.0001, *p < 0.05; one-way ANOVA test. c Upper panel: Western blotting analysis of DNRX expression in neurons and muscle. Bottom panel: Quantitative analysis of DNRX expression levels from the data in the upper panel. The data are presented as the means ± SEMs of three independent experiments. ns, p > 0.05; one-way ANOVA test. d The distance traveled by larvae of DNRX knockdown within 3 min. n = 15, 22, 17, 20, 24, 17, 20 for the different genotypes from elav-Gal4/+ to UAS-dcr;UAS-dnrx IR/+. The data are presented as the means ± SEMs. ****p < 0.0001; ns, p > 0.05; one-way ANOVA test. e Quantification of dnrx mRNA levels in the neurons of DNRX-knockdown flies as indicated. The data are presented as the means ± SEMs of three independent experiments. *p < 0.05; ns, p > 0.05; one-way ANOVA test. f Quantification of dnrx mRNA levels in the muscles of DNRX-knockdown flies as indicated. The data are presented as the means ± SEMs of six independent experiments. **p < 0.01, *p < 0.05; ns, p > 0.05; one-way ANOVA test.
