Fig. 7: DNRX links DG to the RT/TW complex. | Communications Biology

Fig. 7: DNRX links DG to the RT/TW complex.

From: Neurexin facilitates glycosylation of Dystroglycan to sustain muscle architecture and function in Drosophila

Fig. 7

a–a” Immunofluorescence images of HEK293T cells transfected with DG and TW. b–b” Immunofluorescence images of HEK293T cells transfected with DG and RT. c–c” Immunofluorescence images of HEK293T cells transfected with DNRX and TW. d–d” Immunofluorescence images of HEK293T cells transfected with DNRX and RT. e–e” Immunofluorescence images of HEK293T cells transfected with DG, DNRX and RT. f–f” Immunofluorescence images of HEK293T cells transfected with DG, DNRX and TW. Scale bar, 5 μm. a”’–d”’, e””–f”” Plots of the pixel intensity along the straight dashed white lines in the images (a”–d” and e”’–f”’). p: Pearson’s coefficient. g, h Immunoprecipitation demonstrating the interaction between RT and DNRX but not DG in S2 cells. i, j Immunoprecipitation showing that neither DNRX nor DG interacted with TW in S2 cells. k Immunoprecipitation results showing that DNRX, DG, RT, and TW formed a complex in S2 cells.

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