Fig. 3: Effects of acidification and elevated temperature on carbon and nitrogen assimilation of Pocillopora damicornis larvae.

Carbon:nitrogen ratio (C:N) of (a; n = 30) holobiont of the coral Pocillopora damicornis larvae under treatment conditions for five days. Atom percent excess (APE) enrichment of 13C (b; n = 30) and 15N (c; n = 30) assimilation by Symbiodiniaceae, coral host, and holobiont of the coral P. damicornis larvae during the pulse stage. Shaded gray bars indicate the proportion of algal symbiont cells to holobiont enrichment. Treatment conditions: Control (29 °C and pCO2 460 µatm), High pCO2 (29 °C and 1000 µatm), High Temperature (32 °C), and Combined (32 °C and pCO2 1000 µatm). Different lowercase letters indicate statistically significant differences at P < 0.05. Error bars indicate standard errors of the mean. Colors depict the treatment conditions and all black dots depict individual data points.