Fig. 3: Inhibition effect of Flavobacterium isolates against Psa. | Communications Biology

Fig. 3: Inhibition effect of Flavobacterium isolates against Psa.

From: Cross-niche protection of kiwi plant against above-ground canker disease by beneficial rhizosphere Flavobacterium

Fig. 3

a Construction of an evolutionary tree based on OTUs identified from 16S metabarcoding data corresponding to 35 isolates. The numbers in the square on the right indicate the number of isolated, exhibiting a similarity greater than 99% to the OTU sequences. b Morphological characteristics of Flavobacterium isolates in PDA medium. c Antibacterial activities of Flavobacterium OTU1542 (F55), OTU6, OTU337, and OTU193 against Psa. d Phenotypic observations of the control effects of different treatments on Psa-induced bacterial canker in ‘Hongyang’ kiwifruit leaf discs. e Evaluation of the impact of Psa inoculation on the lesion area of ‘Hongyang’ leaf discs following treated/untreated with above Flavobacterium OTUs. f Phenotypic observations of the control effects of different treatments on Psa-induced bacterial canker in ‘Hongyang’ kiwifruit detached canes. g Assessment of the impact of Psa inoculation on the lesion length of ‘Hongyang’ detached canes following treated/untreated with above Flavobacterium OTUs. h, i Phenotypic observations of the control effects of different treatments on Psa-induced bacterial canker in potted ‘Hongyang’ kiwifruit canes and petioles. j, k Assessment of the impact of Psa inoculation on the lesion length of potted ‘Hongyang’ canes and petioles following treatment or no treatment with the specified F55. Psa: negative control, only with GFPuv-labeled M228. Z98: positive control, a pseudomonas strain with antagonistic activity against Psa. Scale bar in (d), (f), (h), and (i) indicates 0.5 cm. Each vertical bar represents the standard error of the means for five replicates. Statistically significant differences (determined by one-way ANOVA with a significance level of P < 0.05) are denoted by lowercase letters.

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