Fig. 3: Pre-incubation with 1-butanol or silencing of PLD decreases HS-activated mTOR.

a Western blot analysis of the phosphorylation states of S6K after HS treatment from 5-d-old WT mycelia. b Western blot analysis of the phosphorylation states of S6K. The mycelia were pre-incubated with 0.3% 1-butanol (1-but), 0.3% 2-butanol (2-but), 100 nM rapamycin (Rapa), or 50 μg/100 mL PA for 30 min before being exposed to HS for 30 min. The histogram in (a) and (b) shows the relative ratio of p-T389-S6K/S6K. p-T389-S6K: phosphorylated S6K at Thr389. c Western blot analysis of the phosphorylation states of S6K after 30 min HS treatment from 5-d-old SiControl and pld-silenced mycelia. The pld-silenced mycelia were incubated with 50 μg/100 mL PA for 30 min before being exposed to HS. d The histogram shows the relative ratio of p-T389-S6K/S6K of (c). The S6K protein level in the WT or SiControl strain under no HS treatments was arbitrarily set to 1. The values are the means ± SD (n = 3 independent experiments). Asterisks in (a) indicate significant differences from the no HS treatments (**P < 0.01 by Student’s t-test); ns not significant. Different letters in (b) and (d) indicate significant differences between the lines (P < 0.05, according to Duncan’s multiple range test).