Fig. 6: SREBP acts downstream of PLD mediated PA interacting protein mTOR in HS-induced GA biosynthesis. | Communications Biology

Fig. 6: SREBP acts downstream of PLD mediated PA interacting protein mTOR in HS-induced GA biosynthesis.

From: Phosphatidic acid directly activates mTOR and then regulates SREBP to promote ganoderic acid biosynthesis under heat stress in Ganoderma lingzhi

Fig. 6

a Exogenous fatostatin (Fato) abolish the total GA content increment elicited by HS in WT strains, and this could not be reverted by PA addition. b The contents of cellular total GA, lanosterol and GA-C2 in SiControl and SREBP-silenced strains. c qRT-PCR analyses of key genes in the GA biosynthetic pathway in SiControl and SREBP-silenced strains. The expression level of each gene from the SiControl strain under no HS treatments was arbitrarily set to 1. a–c 5-d-old WT, SiControl or SREBP-silenced strains were incubated with 10 μM fatostatin (Fato), 50 μg/100 mL PA, 0.3% 1-butanol (1-but) or 100 nM rapamycin (Rapa) before being exposed to HS and then recovered. The values are the means ± SD (n = 3 independent experiments). Different letters indicate significant differences between the lines (P < 0.05, according to Duncan’s multiple range test).

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