Fig. 2: Multiomics uncovers that oral SSa solution modulated hepatic lipid profile.
From: Saikogenin A improves ethanol-induced liver injury by targeting SIRT1 to modulate lipid metabolism
A Scheme of RNA-seq assays and metabolite analysis strategies employing the EtOH and Et+SSa-H group mouse livers (n = 4 in each group). B Volcano plot results of differentially expressed genes and metabolites in the Et+SSa-H vs. EtOH groups. C Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the identified differentially expressed genes and metabolites according to RNA-seq and metabolite data sets from the Et+SSa-H vs. EtOH groups. D Protein level alterations in phosphorylated mTOR, total mTOR, PGC- 1α, PPAR-α, Deptor, and cleaved caspase-3 in the liver from mice containing ALD and/or SSa cotreatment (n = 3). E, F mRNA abundance of SREBP- 1C and PPAR-α possessing their target genes in the liver from mice with ALD and/or SSa cotreatment (n = 6). Data are represented as the mean ± SD, *p < 0.05, **p < 0.01 between the two groups. G Correlation of SIRT1 expression with the expression profiles of genes linked to lipid metabolism in mouse livers according to RNA-seq data. H Combination of GSE28619 and GSE103580 data with batch effect examination of SIRT1 expression. I mRNA abundance of SIRT1 in the liver from mice with ALD and/or SSa cotreatment (n = 6). J Protein level alterations of SIRT1 in the liver from mice with ALD and/or SSa cotreatment (n = 3). K Representative confocal microscopy images (Scale Bar: 20 µm) of immunostaining targeting SIRT1 in mouse liver (n = 3).
