Fig. 5: SIRT1 is associated with oral SSa solution-mediated alleviation of ethanol-induced liver damage.
From: Saikogenin A improves ethanol-induced liver injury by targeting SIRT1 to modulate lipid metabolism
A Schematic knockdown of hepatic SIRT1 in C57BL/6J mice and a timeline of NIAAA mouse model construction. B Representative immunofluorescence microscopy images from liver cryosections (Day 28). Detection of fluorescence signaling of the AAV8 vector with ZsGreen fluorescent tag. C Alterations in hepatic SIRT1 protein levels were assessed in mice with or without hepatic SIRT1 knockdown (Day 50) (n = 3). D Alterations in body weight of mice given SSa with or without hepatic SIRT1 knockdown (n = 6). E The levels of liver/body weight index (n = 6). F, G Representative liver H&E (Scale Bar:60 µm) and Oil Red O staining images from mice with ALD and/or SSa cotreatment, with or without hepatic SIRT1 knockdown (n = 3). H Alterations in plasma biochemical parameters (ALT, AST, TC, TG, and γ-GT) in mice with ALD and/or SSa cotreatment, with or without hepatic SIRT1 knockdown (n = 6). I Changes in hepatic parameters (TC, TG, and NAD+/NADH) in mice with ALD and/or SSa cotreatment, with or without hepatic SIRT1 knockdown (n = 6).
