Fig. 3: Regulation of inflammatory responses in microglia by OGT.
From: KDM2B regulates stroke injury by modulating OGT-mediated 0-GlcNAcylation of SLC7A11
A Immunohistochemical method was used to detect the protein level of Iba1 in brain microglia of MACO mice (scale bar = 20 μm). B Immunofluorescence detection of the expression of CD86 and CD206 in the brain of MACO mice (scale bar = 20 μm). C ELISA was used to detect the expression of pro-inflammatory cytokines IL-1α and THF-α in the brain tissue of MACO mice, n = 6. D ELISA was used to detect the expression of anti-inflammatory cytokines IL-4 and IL-10 in the brain tissue of MACO mice, n = 6. Results are expressed as group mean ± standard deviation. *p < 0.05 vs. Sham group; #p < 0.05 vs. MCAO + NC group. OGD/R and OGT-treated HT-22 medium were used to culture BV2 cells. E The contents of IL-1β and THF-α in the different groups were determined n = 3. F The contents of TGF-β and IL-10 in the different groups were determined n = 3. Results are expressed as group mean ± standard deviation. *p < 0.05 vs. Con group #p < 0.05 vs. OGD/R + NC group.
