Fig. 5: Quantitative analysis of the temporal variations in microvascular parameters at the healing front and in the entire granulation tissue.

a Illustration of the healing front, i.e., the first 0.5 mm from the wound boundary. b Quantification of temporal changes in microvascular sO₂, vessel diameter and tortuosity at the healing front (n = 6 wounds from 4 mice). Baseline measurement was taken on healthy skin of the same group of mice. c Illustration of the granulation tissue, defined as the new tissue that fills the wound. The fresh wound at day 0 was approximated as the white circle with 5 mm diameter located at the geometric center of the wound boundary at each time point. Granulation tissue is the area filling the fresh wound, which enlarges over time. d Quantification of temporal changes in microvascular sO₂, vessel diameter and tortuosity in the entire granulation tissue (n = 6 wounds from 4 mice). Baseline measurement was taken on healthy skin of the same group of mice. Asterisks (*) indicate significant differences (p < 0.05).