Fig. 1: The immune microenvironment of the healthy and inflamed colon characterized by COMET multiplex immunofluorescence and unsupervised clustering analysis.
From: Spatial profiling of the mouse colonic immune landscape associated with colitis and sex
a Profiling of 10 markers in an AOM/DSS-treated colon stained using the COMET platform. Ly-6G (gray), CD8 (yellow), CD3 (magenta), FOXP3 (red), CD86 (green), CD11c (orange), CD4 (purple), Klrb1c (lime), F4/80 (blue), and CD206 (olive). Scale bars: 500 μm (left) and 100 μm (right). b Heatmap generated from unsupervised clustering performed on staining intensities of all cells counted in all samples. The number of cells in each cluster is shown on the left. c tSNE mapping of clusters 2–9 (non-immune cells excluded). d The percentage of cells in clusters 1–9 in all samples grouped by treatment. Vehicle-treated: top. AOM/DSS-treated: bottom. e The percentage of cells in clusters 1–9 from (d) grouped separately by cluster and treatment. Results are given as mean ± SEM and statistical analysis was performed using Mann–Whitney tests (e). f, g The spatial distribution of cells in clusters 1–9 in an AOM/DSS-treated colon sample.
