Fig. 2: The spatial immune landscape of the colon, including interactions between immune cells and sex differences, as characterized by SPIAT.
From: Spatial profiling of the mouse colonic immune landscape associated with colitis and sex
a–c Violin plots showing the distribution of pairwise distances between (a) Treg cells (cluster 2) and DCs (cluster 3), (b) DCs and B cells (cluster 8), and (c) Treg cells and neutrophils (cluster 4). Vehicle-treated samples (blue, left panels), AOM/DSS-treated samples (yellow, right panels), all distances (top panels), 0–20 μm (bottom panels). d–f Images of close interactions identified in (a–c): (d) Treg cells (cluster 2, red) and DCs (cluster 3, orange), (e) DCs and B cells (cluster 8, magenta), and (f) Treg cells and neutrophils (cluster 4, magenta). Scale bars: 20 μm (top) and 10 μm (bottom). g–i Violin plots showing the distribution of pairwise distances in males and females between (g) Neutrophils (cluster 4) and M2-like macrophages (cluster 7) in vehicle-treated samples, and M2-like macrophages and B cells (cluster 8) in (h) vehicle-treated samples and (i) AOM/DSS-treated samples. * indicates P < 0.05.
