Fig. 4: Mucosal immune cell infiltration displays distinct longitudinal differences at homeostasis and in response to colitis.
From: Spatial profiling of the mouse colonic immune landscape associated with colitis and sex
a Graphs visualizing the longitudinal distribution of infiltrating immune cells in the colon at homeostasis (left, vehicle-treated, n = 6) and during colitis (right, AOM/DSS-treated n = 10 mice). b Tc cell and neutrophil infiltration in the different regions of the colon at homeostasis (n = 6 vehicle-treated mice). c Differences in M2-like macrophage, Th cell, NK cell, and neutrophil infiltration over the proximal-distal axis in response to colitis (n = 6 vehicle- and n = 10 AOM/DSS-treated mice). d B cell and Th cell infiltration in regions of squamous metaplasia compared to in different regions of the inflamed colon (n = 9-10 AOM/DSS-treated mice). e Representative images of a region of squamous metaplasia (top part of the image) compared to colonic epithelium (bottom part of the image). Left: Image of H&E-stained section. Right: Image of multiplex immunofluorescence for CD86 (orange), CD3 (blue), and CD4 (magenta). Results are given as mean ± SEM (b–d). Statistical analysis was performed using one-way ANOVA tests with Fisher’s LSD test (b, d) and two-way ANOVA tests with Fisher’s LSD test (c). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Scale bars: 50 μm (e).
