Fig. 1: Carotenoid biosynthesis via the 4,4′-diapophytoene pathway, which includes 4,4′-diapophytoene synthase (encoded by the crtM gene) and 4,4′-diapophytoene desaturase (encoded by the crtN gene).

Theoretical mass of the final product 4,4′-diaponeurosporene is shown (made with ChemDraw and inkskape based on KEGG).