Fig. 6: High Speed Atomic Force Microscope (HS-AFM) imaging of disassembly of Syt-1 clusters in the presence of Ca2+ in real-time.

A Frame-by-frame displays of the dissociation of Syt-1 clusters upon flowing in Ca2+-containing buffer. Most clusters dissociated in one image frame. The interval between neighboring frames was 1 s. B Frame-by-frame displays of the AFM images of the lipid bilayer in the absence of Syt-1 upon flowing in Ca2+-containing buffer.