Fig. 7: Monitoring association and disassociation of two colors of Syt-1 clusters simultaneously. | Communications Biology

Fig. 7: Monitoring association and disassociation of two colors of Syt-1 clusters simultaneously.

From: Quantitative single-molecule analysis of assembly and Ca2+-dependent disassembly of synaptotagmin oligomers on lipid bilayers

Fig. 7

A Schematic illustration of dissociation of wildtype Syt-1 Alexa488 clusters and formation of wildtype Syt-1 Alexa647 clusters. Syt-1 Alexa488 clusters on lipid bilayer were pre-formed by incubating with Syt-1 Alexa488 solution. Then Syt-1 Alexa647 solution flowed in. B Representative TIRF images of Syt-1 Alexa 488 clusters (top panel, green), and Syt-1 Alexa647 clusters (bottom panel, white), at different time. The two panels showed the same area on the lipid bilayer. Growth of new clusters usually occurred at a fresh spot (n = 3).

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