Fig. 2: Rbm4 knockout affects cassette exon selection and causes intron retention.
From: RBM4-mediated intron excision of Hsf1 induces BDNF for cerebellar foliation
A RT-PCR of selected RBM4 targets in the CES category from E13.5 wild-type (WT) and Rbm4dKO (KO) brains. Bar graphs show relative PSI (+e/total); WT was set to 1 (n = 3). GAPDH was used as a loading control. B RT-PCR of selected RBM4 targets in the IR category from E13.5 brains. Bar graphs show relative PIR (+i/total); WT was set to 1 (n = 3). P-values were determined using the Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001. The number in parentheses represents basepair (bp). C Immunoblotting shows three selected proteins (HSF1, hnRNPH1, hnRNP L) in E13.5 WT and KO brain lysates. The level of examined proteins was normalized to GAPDH. Numbers below the blots indicate relative protein level (WT was set to 1; n = 3). P-values are listed in Supplementary Data 2.
