Fig. 4: HSF1 rescues the foliation defects of the Rbm4 knockout cerebellum.
From: RBM4-mediated intron excision of Hsf1 induces BDNF for cerebellar foliation
A Schematic diagram shows the construct of the DsRed (Con) and HSF1-DsRed fusion, and the procedure of in utero electroporation (IUE). The empty or HSF1-DsRed plasmid was electroporated into the lateral ventricle of the E15.5 Rbm4dKO brain. Newborn pups (Rbm4dKOCon and Rbm4dKOHSF1) were sacrificed at P0 or P30. B The expression levels of BDNF mRNA and indicated protein were evaluated in the P0 cerebellum of wild-type (WT), Rbm4dKO, Rbm4dKOCon, and Rbm4dKOHSF1. C Hematoxylin and eosin staining were performed in P0 and P30 cerebellums of mice as indicated. Arrowhead indicates icf. D The icf depth was measured in the P30 cerebellum. Bars represent average ± SEM (n = 3, ***P < 0.001). E For rotarod analysis, each mouse (6-week-old) was trained on the rod for 60 s at low constant speed (Supplementary Fig. 3C) before proceeding to the tests with an accelerating rotational speed from 4 to 40 rpm over a period of 300 s. Bar graph shows the average latency to fall (s) (10 mice for each group); ***P < 0.001; **P < 0.01.
