Fig. 5: NMDA receptor signaling regulates RBM4-mediated intron excision of Hsf1.
From: RBM4-mediated intron excision of Hsf1 induces BDNF for cerebellar foliation
A GCs were treated with different concentrations of NMDA for one hour. RT-PCR of Hsf1 and immunoblotting of HSF1, BDNF, and GAPDH were performed. PIR (%) and relative protein level were measured as in Fig. 3A. Bar graph shows Hsf1 introns 5/6/7 PIR of 25 µM NMDA-treated (+) relative to mock-treated (−) cells (***P < 0.001; n.s. no significance). B GCs were transfected with the control or Rbm4 shRNA followed by NMDA (25 µM) or DCS (50 μM) treatment. RT-PCR of Hsf1 was performed. Lane number is indicated below the gel. C–F GCs were mock-treated (−) or pre-treated with AP5 (25 μM), EGTA (2 mM), CK59 (500 nM) or SRPIN340 (30 µM) followed by NMDA treatment. RT-PCR, immunoblotting, and measurement of PIR (%) and relative protein levels were performed as in (A). G Schematic diagram shows that NMDAR signaling modulates RBM4-mediated Hsf1 intron 6 splicing. Activators and inhibitors of NMDAR signaling pathways are indicated in red and green ovals, respectively.
