Fig. 1: Kcnb1^R312H(+/+) mice are hyperactive.

A Heat maps showing 24 h of activity of single-housed WT and Kcnb1^R312H(+/+) males and females. The period of major activity begins at ∼ 18 h, when the light is turned off, and terminates at ∼6 h, when the light is turned on. N = 7 mice/sex/genotype, recorded over a week. B Distance traveled per minute, during the day, averaged over a week for WT and Kcnb1^R312H(+/+) males and females. N = 28 mice (seven males and seven females per genotype). C Cumulative daily distance traveled over a week by WT and Kcnb1^R312H(+/+) males and females. N = 28 mice (seven males and seven females per genotype). D Cumulative distance run on the running wheel over a week by WT and Kcnb1^R312H(+/+) males and females combined. N = 5 mice (three males and two females per genotype). In all experiments, three-month-old mice were used. There were no differences in size and body weight between WT and Kcnb1^R312H(+/+) mice. ^*P < 0.05, ^***P < 0.001, two-tailed student’s t-test.