Fig. 3: The brain of Kcnb1^R312H(+/+) mice shows diffuse neuronal damage.

A Representative confocal visualizations of neocortical neurons co-immunostained with NFM (green) and L1CAM (magenta) in the indicated areas of the brain (cortex CTX, corpus callosum CC, cingulum Cing, nucleus habenular nucleus hab, fimbria Fimbriae, CA3 region of the hippocampus: CA3) in WT and Kcnb1^R312H(+/+) brain slices. DAPI (blue color). Scale bars CTX: 200 μm. Others: 100 μm. B–F Quantifications of the mean neocortex thickness (B) and of individual layers (from left to right, C layer I, D layers II–III, E layer IV, and F layers V–VI) in WT and Kcnb1^R312H(+/+) (R312H) slices. G Part of the whole representations of the thickness of the cortical layers in WT and Kcnb1^R312H(+/+) brain slices. H Quantifications of the mean CC bundle of WT and Kcnb1^R312H(+/+). I As in (H) for the mean cingulum-CC bundle. J As in (H) for the mean cingulum bundle height. K As in (H) for the mean fimbria width. L As in (H) for the habenular nuclei. M As in (H) for the fibers of the CA3 region of the hippocampus. Slices were cut from three-month-old brains/genotypes at Bregma coordinates −1.46 mm/−1.58 mm (mouse brain atlas⁸²). ^*P < 0.05 and ^**P < 0.01, two-tailed student t-test.