Fig. 5: Similar firing patterns in WT and Kcnb1^R312H(+/+) primary neurons.

A–D Passive membrane properties of WT and Kcnb1^R312H(+/+) primary neurons: A rheobase; B resting membrane potential (RMP), C capacitance, and D input resistance. E Mean height of the action potential (AP) fired by WT or Kcnb1^R312H(+/+) neurons. F Mean half width of the AP fired by WT or Kcnb1^R312H(+/+) neurons. G Spike frequency as a function of input current for WT and Kcnb1^R312H(+/+) neurons. N = 26 cells/genotype. H Representative current-clamp recordings of the electrical activity of a WT and a Kcnb1^R312H(+/+) primary neuron in the absence/presence of 100 nM GxTx. Firing activity was triggered by a 100 pA current injection. I Spike frequency induced by 50 pA current injection in WT, Kcnb1^R312H(+/+), and Kcnb1^NULL(+/+) neurons in the absence/presence of 100 nM GxTx in the bath. All recordings were performed using DIV14 neurons, obtained from N = 7 WT brains, N = 7 Kcnb1^R312H(+/+) brains, and N = 5 Kcnb1^NULL(+/+) brains with three dishes/brain. ^*P < 0.05, two-tailed student’s t-test.