Fig. 6: Dysregulated glutamatergic vesicular transport in the Kcnb1R312H(+/+) brain. | Communications Biology

Fig. 6: Dysregulated glutamatergic vesicular transport in the Kcnb1R312H(+/+) brain.

From: Abnormal cytoskeletal remodeling but normal neuronal excitability in a mouse model of the recurrent developmental and epileptic encephalopathy-susceptibility KCNB1-p.R312H variant

Fig. 6

A Representative confocal tile images of WT and Kcnb1R312H(+/+) coronal brain sections showing overlap of VGluT1, VGluT2 and DAPI and heat maps of VGlut1 or VGluT2 fluorescence (blue is the lowest fluorescence). Scale bars 200 μm. B Magnifications of the heat maps of immunostainings in (A) showing VGluT2 expression in the various cortical parts. Insets: magnifications of the barrel cortex (S1). C Quantifications of VGluT1 and VGluT2 immunostaining in the various areas of the brain. CX cortex, Hipp hippocampus, Hb habenular nuclei, Amyg amygdala, Tha thalamus, Hl hypothalamus. N = 3 three months old brains/genotype. *P < 0.05, two-tailed student’s t-test.

Back to article page